OCR A Level Biology A
OCR A Level Biology A Manipulating Genomes Exam Questions
Free OCR A Level Biology A exam questions on Manipulating Genomes, with mark schemes. Covers DNA sequencing, PCR, gel electrophoresis, genetic profiling, genetic engineering and gene therapy.
Manipulating Genomes exam questions
1 question pack of real past exam questions with its mark scheme. Answer every question without notes, then mark your answers line by line against the mark scheme.
StemUp mark scheme flashcards
OCR A Level Biology A H420 Flashcards (Anki & Quizlet)
Every answer is written in mark scheme wording, so you learn exactly what examiners give marks for. Use them on Anki or Quizlet, then test yourself with the questions on this page.
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About OCR A Level Biology A Manipulating Genomes
This topic covers DNA technology.
What you need to know
- Sequencing Sanger and high-throughput sequencing and bioinformatics.
- PCR and electrophoresis Amplifying and separating DNA fragments.
- Genetic engineering Restriction enzymes, ligase, vectors and transformation.
- Gene therapy Somatic and germ line therapy and ethical issues.
Common mistakes in Manipulating Genomes
- Saying PCR uses human DNA polymerase.
- Forgetting DNA is negative in electrophoresis.
- Mixing somatic and germ line therapy.
Try three Manipulating Genomes exam-style questions
1. State the three temperatures in PCR. (2 marks)
Show the mark scheme answer
About 95 °C to separate strands (1), 55 °C for primers to anneal and 72 °C for extension (1).
2. Explain why DNA moves to the anode. (1 mark)
Show the mark scheme answer
Phosphate groups give DNA a negative charge.
3. Give one ethical concern of germ line therapy. (1 mark)
Show the mark scheme answer
Changes are passed on to future generations without consent.
Manipulating Genomes FAQs
What does this topic cover?
Sequencing, PCR, electrophoresis and genetic engineering.
Which practical links here?
Gel electrophoresis.
What is a vector?
A carrier, such as a plasmid, used to insert DNA into a host.
Revise this topic with flashcards: OCR A Level Biology A H420 Flashcards, with mark scheme answers for Anki and Quizlet.
